
Is it important to avoid microRNA binding sites during siRNA design?
Summary: To address the question of whether one should avoid microRNA binding sites during siRNA design, we examined whether removing siRNAs that share seeds with native microRNAs would reduce the dominance of seed-based off-target effects in RNAi screening. siRNA design and native microRNA target sites Recently, we discussed a review of genomics screening strategies. The authors state: RNAi screens are powerful and readily implemented discovery tools but suffer from shortcomings arising from their high levels of false negatives and false positives (OTEs) Read More