
Comparing silencing for CDS and 3′ UTR siRNAs
CDS vs. 3′ UTR In some cases, it may be preferable to only target the 3′ UTR of an mRNA. For example, the CDS may be highly similar to a related paralog gene that should not be co-targeted. Or a rescue experiment will be performed, and one would rather use the native CDS for rescue. (Note that we can also provide rescue constructs for siPOOLs that target CDS. The rescue sequence uses the most common alternative codon in the targeted Read More